![]() as per Annexure-II.ĥ.2.5 Dispense the medium in an individual container as per requirement plug the containers with a cotton plug or screw cap.ĥ.2.6 Sterilize the Media for 20 minutes at 121☌ & 15 psi or for the validated time period.ĥ.3 Growth promotion test of sterilized media.5.3.1 Select the quantified microbial culture as per Annexure –I, for the growth promotion testĥ.3.2 Quantified culture should be 24 hours old or a validated period old microbial culture can be used for growth promotion having count 10-100 cfu/ml.ĥ.3.3 For Growth promotion test of the agar medium, transfer microbial culture to sterile Petri dishes in duplicate aseptically and pour 15 to 20 ml of agar medium (maintain the temperature of the medium before pouring at 40-45☌)ĥ.3.4 Incubate the Petri plates for 24 - 48 hours for the bacterial count at 30☌-35☌ and 5 to 7 days for the fungal count at 20☌-25☌.ĥ.3.5 After completion of incubation period count the colonies of microorganisms. date, Date of preparation, Prepared by, pH before sterilization and after sterilization (Broth medium), the volume of media.etc. Make up the volume with remaining quantity of water.ĥ.2.2 Check the pH of media using calibrated pH meter, adjust the pH using 0.1M HCl/0.1M NaOH if required.ĥ.2.3 Follow all instructions/ precautions of the manufacturer at the time weighing and re-hydration of media. Weight the quantity of the dehydrated media as per volume required, transfer in a conical flask and re-hydrate it as per manufacturer instructions. Transfer the half of the volume of purified water of required quantity in the conical flask. Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).1.0 OBJECTIVETo lay down a procedure for media preparation and growth promotion test.Ģ.0 SCOPEThis procedure is applicable for media preparation and growth promotion test for microbial analysis.ģ.0 RESPONSIBILITYMicrobiologist - Quality ControlĤ.0 ACCOUNTABILITYManager - Quality Controlĥ.0 PROCEDURE 5.1 General precautions5.1.1 Take clean, dried glass conical flask of the desired volume as per Quantity of media.ĥ.1.2 Use a separate spatula for separate media to avoid cross contamination.ĥ.1.3 Clean the balance in between to successive weighing.ĥ.1.4 Use purified water for media preparation.ĥ.1.5 Media preparation and media discard activities will perform at different location/Timeĥ.2 Preparation of media5.2.1 Take clean dried conical flask as per the requirement of media.TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products. ![]() TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Trypticase Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens. ![]()
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